Table 1.

Assessment of c-Myc gene amplification levels in different cell types.

Method	c-Myc genomic copy number	c-Myc amount exoRNA*	c-Myc amount exoDNA*
D425
FISH†	> 25
250K SNP‡	15
qPCR	8 ± 3.6	8 ± 2.0	13 ± 0.2
D384
250K SNP	25
qPCR	12 ± 4.7	42 ± 22	25 ± 3.7
D458
250K SNP	17
qPCR	17 ± 3.0	45 ± 11	10 ± 0.6
NS224
250K SNP	2
qPCR	2	0.8 ± 0.3	4.2 ± 0.1
GBM11/5
qPCR	2	2.8 ± 1.4	0.4 ± 0.1
HF19
qPCR	2	1	1

^*Reverse transcribed exoRNA (2.5 ng) and exoDNA (10 ng) were used as template for qPCR. All values were normalized to GAPDH mRNA.

^†FISH, Fluorescence in situ hybridization of metaphase chromosome spread⁶⁰.

^‡See representative heat map Supplementary Figure S5c.