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. 2011 Jan 26;4:18. doi: 10.1186/1756-0500-4-18

Figure 2.

Figure 2

Dynamics of E. coli before and after induction with IPTG. A. Growth profile of E. coli M15 cells. Panels a, b, c and d show growth profiles of pQE30/M15, OsGR-RBP4/M15, OsGR-RBP4ΔC/M15 andOsFKBP0/M15 E. coli cell types, respectively. Purple line denotes un-induced and blue line denotes induced E. coli M15 cells. B. a. Plate culture assay of OsGR-RBP4/M15, OsGR- RBP4PΔC/M15, OsFKBP20/M15 and pQE30/M15 E. coli cells (incubation at 37°C). Cells were harvested at various time points after IPTG induction as shown. Serial dilutions were made and equal number of cells was spotted on LB agar plates with antibiotic selection. Plates were photographed after 16 h of incubation. +IPTG refers to induction by IPTG for the time periods indicated at the top. b. Plate assay of OsGR-RBP4/M15, OsGR-RBP4PΔC/M15, OsFKBP20/M15 and pQE30/M15 E. coli cells (incubation temperature of the plates = 20°C). Cells were harvested at various time points after IPTG induction as shown. c. Plate assay of OsGR-RBP4/M15, OsGR-RBP4PΔC/M15, OsFKBP20/M15 and pQE30/M15 E. coli cells, (incubation temperature of the plates = 45°C).