Table 3.
List of proteins that showed a decreased (down) or an increased (up) trend (p > 0.05) in the lesion center of the injured spinal cord from the subacute (day 14) SCI group when compared to that detected in the acute (day 1) SCI group.
| Spot no. |
Function | Protein name | Protein ID | Expression (14d/1d) |
14d_mean (SEM) |
1d_mean (SEM) |
p value | Mw/pI | Score | |
|---|---|---|---|---|---|---|---|---|---|---|
| 119 | actin filament binding | Fascin | FSCN1 | down | 0.053 (0.022) |
0.106 (0.046) |
0.406 | 54474/6.44 | 184 | |
| 6,7 | anti-apoptosis | 60 kDa heat shock protein, mitochondrial | CH60 | down | 0.123 (0.050) |
0.744 (0.340) |
0.052 | 61088/5.91 | 96 | |
| 11,12 | isomerase | Protein disulfide-isomerase A3, p58 | PDIA3 | down | 0.618 (0.188) |
1.386 (0.955) |
0.344 | 57044/5.88 | 80 | |
| 13,14 | metabolism | D-3-phosphoglycerate dehydrogenase | SERA | down | 0.096 (0.081) |
0.231 (0.087) |
0.355 | 56457/6.28 | 90 | |
| 38 | metabolism | Acetyl-CoA acetyltransferase, cytosolic | THIC | down | 0.052 (0.009) |
0.189 (0.112) |
0.272 | 41538/6.86 | 60 | |
| 44,45 | metabolism | Fructose-bisphosphate aldolase A | ALDOA | down | 1.256 (0.239) |
1.905 (0.570) |
0.292 | 39783/8.31 | 125 | |
| 46 | metabolism | L-lactate dehydrogenase B chain | LDHB | down | 0.396 (0.072) |
1.092 (0.613) |
0.283 | 36874/5.70 | 71 | |
| 99 | metabolism | Malate dehydrogenase, cytoplasmic | MDHC | down | 0.240 (0.087) |
0.648 (0.238) |
0.121 | 36117/8.93 | 217 | |
| 100, 101 | metabolism | Carbonic anhydrase 2 | CAH2 | down | 0.053 (0.025) |
0.484 (0.228) |
0.071 | 29096/6.89 | 76 | |
| 109 | metabolism | Glycine amidinotransferase, mitochondrial | GATM | down | 0.032 (0.011) |
0.052 (0.016) |
0.416 | 48724/7.17 | 146 | |
| 113~ 115 | metabolism | Aconitate hydratase, mitochondrial | ACON | down | 0.164 (0.058) |
0.712 (0.323) |
0.102 | 86121/7.87 | 183 | |
| 39 | metabolism | Creatine kinase M-type | KCRM | down | 0.066 (0.020) |
0.101 (0.032) |
0.391 | 43246/6.58 | 63 | |
| 30,31 | metabolism | Phosphoglycerate kinase 1 | PGK1 | down | 0.452 (0.123) |
0.803 (0.468) |
0.446 | 44909/8.02 | 116 | |
| 9,10 | microtubule | Tubulin alpha-1B chain | TBA1B | down | 1.111 (0.488) |
1.941 (0.876) |
0.370 | 50120/4.94 | 86 | |
| 89 | microtubule | Tubulin alpha-1A chain | TBA1A | down | 0.146 (0.039) |
0.279 (0.062) |
0.102 | 50788/4.94 | 135 | |
| 88,90, 91 | neurogenesis | Dihydropyrimidinase-related protein 2 | DPYL2 | down | 0.176 (0.088) |
0.313 (0.116) |
0.366 | 62638/5.95 | 105 | |
| 92~94 | neuron differentiation | Dihydropyrimidinase-related protein 5 | DPYL5 | down | 0.061 (0.046) |
0.319 (0.189) |
0.132 | 61501/6.60 | 117 | |
| 105 | oxidoreduction | Dihydrolipoyl dehydrogenase, mitochondrial | DLDH | down | 0.151 (0.069) |
0.376 (0.165) |
0.214 | 54574/7.96 | 84 | |
| 95 | oxidoreduction | Catalase | CATA | down | 0.028 (0.027) |
0.056 (0.013) |
0.364 | 59719/7.07 | 187 | |
| 82,83 | protease inhibitor | Serine protease inhibitor A3K | SPA3K | down | 0.299 (0.078) |
0.866 (0.289) |
0.073 | 46532/5.31 | 113 | |
| 15 | protein assembly | Stress-induced-phosphoprotein 1, Hsc70/Hsp90-organizing protein | STIP1 | down | 0.035 (0.020) |
0.172 (0.139) |
0.288 | 63158/6.40 | 65 | |
| 17 | proteolysis | Cytosol aminopeptidase | AMPL | down | 0.122 (0.040) |
0.188 (0.064) |
0.400 | 56514/6.77 | 62 | |
| 120 | stress response | Heat shock cognate 71 kDa protein | HSP7C | down | 0.391 (0.257) |
0.865 (0.332) |
0.288 | 71055/5.37 | 123 | |
| 119 | actin filament binding | Fascin | FSCN1 | down | 0.053 (0.022) |
0.106 (0.046) |
0.406 | 54474/6.44 | 184 | |
| 63 | anti-apoptosis | Lactoylglutathione lyase | LGUL | up | 0.488 (0.078) |
0.231 (0.090) |
0.074 | 20977/5.12 | 67 | |
| 62 | cell proliferation | Translationally-controlled tumor protein | TCTP | up | 0.181 (0.051) |
0.058 (0.057) |
0.216 | 19564/4.76 | 128 | |
| 8 | chaperone | Protein disulfide-isomerase | PDIA1 | up | 0.897 (0.253) |
0.263 (0.099) |
0.097 | 57315/4.82 | 197 | |
| 67 | chaperone | Protein DJ-1 | PARK7 | up | 0.260 (0.087) |
0.113 (0.034) |
0.198 | 19961/6.32 | 68 | |
| 124 | chaperone | T-complex protein 1 subunit beta | TCPB | up | 0.078 (0.015) |
0.031 (0.030) |
0.190 | 57422/6.01 | 76 | |
| 21 | metabolism | Elongation factor 1-alpha 1 | EF1A1 | up | 0.631 (0.273) |
0.160 (0.055) |
0.142 | 50424/9.10 | 77 | |
| 29 | metabolism | Isocitrate dehydrogenase [NADP] | IDHC | up | 0.113 (0.111) |
0.056 (0.044) |
0.679 | 47047/6.53 | 108 | |
| 50 | metabolism | L-lactate dehydrogenase A chain | LDHA | up | 0.209 (0.045) |
0.080 (0.028) |
0.058 | 36712/8.45 | 64 | |
| 53 | metabolism | Dimethylarginine dimethylaminohydrolase 2 | DDAH2 | up | 0.118 (0.044) |
0.015 (0.014) |
0.158 | 30011/5.66 | 116 | |
| 73,75 | oxidoreduction | Peroxiredoxin-1 | PRDX1 | up | 0.684 (0.378) |
0.076 (0.036) |
0.158 | 22323/8.27 | 91 | |
| 65 | oxidoreduction | Peroxiredoxin-6 | PRDX6 | up | 0.199 (0.104) |
0.074 (0.023) |
0.359 | 24860/5.64 | 74 | |
| 79 | protein assembly | 78 kDa glucose-regulated protein | GRP78 | up | 0.323 (0.116) |
0.136 (0.045) |
0.245 | 72473/5.07 | 285 | |
| 51 | proteolysis | Cathepsin B | CATB | up | 0.245 (0.081) |
0.159 (0,158) |
0.619 | 38358/5.36 | 72 | |
| 106, 107 | proteolysis | Cathepsin D | CATD | up | 0.840 (0.373) |
0.036 (0.026) |
0.075 | 45165/6.66 | 132 | |
| 64 | signal transduction | Phosphatidylethanolamine-binding protein 1 | PEBP1 | up | 1.539 (0.440) |
0.910 (0.214) |
0.277 | 20902/5.48 | 82 | |
| 72 | signal transduction | GTP-binding nuclear protein Ran | RAN | up | 0.184 (0.090) |
0.116 (0.055) |
0.606 | 46532/5.31 | 113 | |
| 57 | stress response | Endoplasmic reticulum protein ERp29 | ERP29 | up | 0.118 (0.091) |
0.029 (0.029) |
0.513 | 28614/6.23 | 63 | |
| 56 | ubiquitin-dependent protein catabolism | Proteasome subunit alpha type-1 | PSA1 | up | 0.177 (0.038) |
0.091 (0.027) |
0.162 | 29784/6.15 | 64 |