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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: Cell Cycle. 2010 Feb 12;9(4):736–747. doi: 10.4161/cc.9.4.10613

Figure 4.

Figure 4

Dissociation of CHK2 and PP2A B’α is dependent on ATM activity, but not PP2A activity. (A) 293T cells were transiently transfected with FLAG-B’α, treated with 0.5 μM oA for 1 h and then irradiated with 20 Gy IR and incubated for 1 h. Whole cell lysates were immunoprecipitated with the FLAG antibody and immunoblotted for the indicated proteins. (B) 293T cells were transiently transfected with FLAG-B’α, treated with 20 μM wortmannin for 1 h then irradiated with 10 Gy IR and incubated for 1 h. Whole cell lysates were immunoprecipitated with the FLAG antibody and immunoblotted for the indicated proteins. (C) HCT116 CHK2−/− cells were transfected with FLAG-B’α and either wild-type CHK2 or the S33/35A or S33/35E mutants. Whole cell lysates were immunoprecipitated with the FLAG antibody and immunoblotted for CHK2.