Figure 3. Effect of silicone rinses on barnacle glue trypsin activity.

30 second, 60 µl methanol rinses were conducted, 10 rinses were pooled, dried completely, and residual was resuspended in 10 µl 100% methanol before adding assay buffer. Each individual barnacle was tested with all four silicones. Individual data, expressed as percent change in OD₄₀₅ from control, and group mean (± SEM) are shown. The control is barnacle glue incubated with 10 µl 100% methanol and assay buffer only, without silicone residual.