Figure 7. Effect of silicone oil and PDMS oligomers on purified trypsin and transglutaminase activity from porcine and guinea pig, respectively.

Silicone oil (viscosity 40–50 cSt) and low, medium and high molecular weight PDMS oligomers (viscosity 700–800, 1000, and 5000 cSt respectively) were tested alone and in combination. Components were dissolved in methanol, the methanol was then dried completely, and residual was resuspended directly in assay buffer. Data are expressed as percent change in OD₄₀₅ (trypsin) or OD₄₅₀ (transglutaminase) from control. Means and SEM are shown. The control is purified enzyme incubated with assay buffer only, without silicone components. * Indicates a significant difference from control (Dunn's method post-hoc analysis: p<0.05). n = 10 replicates for individual components, 5 replicates for combinations.