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. 2011 Feb 17;6(2):e14712. doi: 10.1371/journal.pone.0014712

Figure 2. Inhibitory effects of E. coli Nissle 1917 on Salmonella Typhimurium invasion is dependent on adhesion.

Figure 2

Confluent monolayers of IPEC-J2 cells were pre-incubated with E. coli Nissle 1917 (EcN), EcN ΔfocA, EcN Δfim or EcN ΔfliA using an MOI of 100∶1 E. coli to host cells. After two or six hours, cells were washed and infected with Salmonella Typhimurium using an MOI of 100∶1 Salmonella to host cells. Invasion levels in percent (%) are expressed as invasion of Salmonella relative to invasion without pre-incubation with E. coli (Salmonella mono-infection). The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells. * = p<0.01 compared to Salmonella mono-infection. A) Effects of EcN ΔfocA and EcN Δfim mutants on Salmonella invasion after a 2 or 6 hours pre-incubation period. B) Effects of EcN ΔfocA and EcN ΔfliA mutants and their respective strains complemented with the plasmid pACYC 177 containing the relevant gene on Salmonella invasion after 2 hours pre-incubation.