Table 1. Survival of PTBP1 null mutants.
genotype | ratio PCR | ratio IF | |||||
age | method | wt | het | hom | wt/het | hom/het | hom/het+wt |
E6.5 | IF | 19 | 5 | 0.26 | |||
E7.5 | PCR | 5 | 12 | 5 | 0.42 | 0.42 | |
E7.5 | IF | 3 | 3 | 1.00 | |||
E8.5 | IF | 7 | 2 | 0.29 | |||
E12.5 | PCR | 8 | 17 | 0 | 0.47 | n/a | |
E16.5 | PCR | 1 | 3 | 0 | 0.33 | n/a | |
3 weeks | PCR | 25 | 44 | 0 | 0.57 | n/a | |
expect: | 0.50 | 0.50 | 0.33 |
The table summarises the genotype of embryos during gestation as determined by PCR or immunofluorescence (IF), the latter of which cannot distinguish between wild-type (wt) and heterozygous (het). The last viable homozygous (hom) embryos were detected at day 8.5 of embryonic development (E8.5). No homozygous mutants were found at E12.5 or later. Genotype ratios observed and expected are shown on the right. While they are close to the expected Mendelian ratio for experiment with several litters, random variations and deviation from the expected ratios are larger in single litter experiments.