Table 1. Knockdown of hCdc14B leads to increase of the mitotic index, multinucleation and apoptosis.
Release from G1/S (h) | siCtrl: mitotic index (%) | siCdc14B-3: mitotic index (%) |
8 | 9.4±2.7 | 8.6±2.5 |
10 | 11.3±3.8 | 11.0±4.7 |
12 | 56.6±6.8 | 51.4±12.8 |
14 | 28.3±4.2 | 50.5±10.6 |
16 | 16.9±4.0 | 47.9±8.8 |
HeLa Kyoto cells transfected with hCdc14B-specific siRNA-3 (siCdc14B-3) or non-targeting siRNAs (siCtrl) were synchronized as shown in Fig. 3A and B. Mitotic cells with condensed chromatin were counted 8–16 h after release from G1/S, multinucleated cells were counted 8 h after release, and apoptotic cells were visualized by TUNEL staining 24 h after release. Numbers represent the mean levels (±SD) from three independent experiments comprising counts of 400 cells each.