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. 2011 Feb 17;6(2):e16550. doi: 10.1371/journal.pone.0016550

Figure 1. Chaperone levels in AD model mice.

Figure 1

(A) Immunoblots showing age-dependence of αB-crystallin expression in mice expressing mutant human APP transgene. Brain lysates were prepared from two individual non-transgenic (NTg1 & NTg2) or transgenic (Tg1 & Tg2) mice each at 3 months or 7 months of age. Samples were immunoblotted for αB-crystallin, Hsp70, Hsp27, Aβ and actin. 40 µg of total protein was analyzed. Similar levels of Hsp70, Hsp27 and actin in all samples show equal protein loading. (B) Densitometric analysis of band intensities in A. Intensities were compared by two-sample Student's t-test for statistical signifcance. APP expression in Tg mice were significantly greater than NTg mice as expected (p = 0.006 at 3 months, p = 0.017 at 7 months). αB-crystallin expression was significantly lower at 7-months compared to 3-months old transgenic mice brain (p = 0.044). αB-crystallin levels in 7-month old transgenic mice was also significantly lower than non-transgenic mice at similar age (p = 0.018). All other comparisons showed no significant differences.