Table 3. Summary of the primary antibodies used in this study.
| Antibody(clone number) | Source | Optimal dilution | Antigen retrieval |
| Human Vimentin(monoclonal: 280618) | R&D (Minneapolis, MN, USA) | 1∶50 | no retrieval |
| Human Cytokeratin(monoclonal: KL1) | AbD serotec (Oxford, UK) | 1∶50 | heata, EDTA·Tris (pH9.0) |
| Human MMP-1(monoclonal: EP1247Y) | Epitomics (Burlingame, CA,USA) | 1∶100 | autoclaveb, citric acid buffer |
| Human MMP-2(polyclonal) | Zhongshan Biotechnology (Beijing, China) | 1∶150 | autoclaveb, citric acid buffer |
| Human MMP-9(polyclonal) | Zhongshan Biotechnology (Beijing, China) | 1∶150 | heata, citric acid buffer |
| Human CD31(monoclonal: JC70A) | Dako (Glostrup, Denmark) | 1∶40 | heata, EDTA (pH8.0) |
| Mouse CD31(monoclonal: MEC13.3) | Biolegend (San Diego, CA, USA) | 1∶100 | trypsinc |
| Human CD45(polyclonal) | Zhongshan Biotechnology (Beijing, China) | 1∶200 | heata, citric acid buffer |
| Mouse CD45(monoclonal:30-F11) | BD Pharmingen(San Diego, CA, USA) | 1∶150 | heata, citric acid buffer |
| Prolactin(monoclonal: C-17) | Santa Cruz (CA, USA) | 1∶500 | heata, citric acid buffer |
a
heat: heat without boiling for 15 min.
b
autoclave: heat in an autoclave for 2 min in citric acid buffer.
c
trypsin: enzymatic digestion by trypsin for 10 min.