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. 2011 Feb 17;6(2):e16871. doi: 10.1371/journal.pone.0016871

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Geiger et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) RIE-1 cells expressing mutant or wild-type TRKB were transduced with BDNF and analyzed on immunoblot (IB). Arrowhead indicates position of BDNF. Tubulin serves as loading control. (B) Morphologic transformation of RIE-1 cells co-expressing mutant or wild-type TRKB and BDNF, photographed at 50x magnification. (C) The epithelial marker E-cadherin is downregulated in TRKB-induced, morphologically transformed, cells, as assessed by immunoblot (IB) analysis. β-actin serves as loading control. (D) Anoikis suppression by mutant or wild-type TRKB+BDNF in cells described in (A). Cells were scanned at 1x magnification 9 days after seeding onto ULC plates.