Figure 5. Oncogenic potential of human cancer-derived TRKB mutants expressed in RIE-1 cells.

(A) 1*10⁶ RIE-1 cells expressing TRKB (wild-type or mutant) were subcutaneously injected into both flanks of nude mice. n = 5 for wt and P507L, n = 4 for T695I and D751N. (B) 1*10⁶ RIE-1 cells expressing TRKB (wild-type or mutant) were subcutaneously injected into both flanks of nude mice. n = 4 for both cell lines. (C) 1*10⁴ RIE-1 cells co-expressing BDNF and TRKB (wild-type or mutant) were subcutaneously injected into nude mice. n = 6 for wt and P507L, n = 3 for T695I and D751N. (D) 1*10⁵ RIE-1 cells co-expressing BDNF and TRKB (wild-type or mutant) were subcutaneously injected into nude mice. n = 4 for both cell lines. In all experiments, mice were sacrificed when tumor burden reached 2 cm² and Kaplan-Meier survival curve is shown. Statistical significance was determined with a Log-Rank test.