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. 2011 Feb 17;6(2):e17124. doi: 10.1371/journal.pone.0017124

Figure 5. FGF and TGF-β signalings are essential for rabbit TS-like cells self-renewal.

Figure 5

(A) RT-PCR detected the mRNA expressions of the key components of FGF and TGF-β signalings in TS-like cells. Lane 1, sample of rabbit ES cells; Lane 2, sample of TS-like cells at 17th passage; Lane 3, sample of TS-like cells at 40th passage. (B) FACS examination of cell cycle stages (G1, G2, and S phases) after TS-like cells were treated with growth factors for 48 hours in the presence or absence of serum. Note that in the absence of serum, growth factors stimulate cell proliferation (n = 3, * represents P<0.05). (C) FACS examination of cell cycle stages (G1, G2, and S phases) after TS-like cells were treated with growth factor inhibitors (20 µM of SB431542 against TGF-β type I receptor or 20 µM SU5402 against FGF receptor 1) for 48 hours in the serum-free medium (SF). Note that growth factor inhibitor treatments increased the percentages of TS-like cells at G1 phase (n = 3, * represents P<0.05). (D) Semi-quantitative RT-PCR analysis of mRNA expressions of the genes specific to trophoblastic lineage in TS-like cells cultured in ES medium (ES), ES medium with TGF-β (ES+TGF-β), serum free medium (SF), serum free medium with SB431542 (SF+SB), or serum free medium with SU5402 (SF+SU).