Figure 8. Native PAGE showing the HmuY-haem complex formed through co-incubation of oxyhaemoglobin with HRgpA plus HmuY.

Oxyhaemoglobin was co-incubated with HmuY plus HRgpA (CBB stained gel A and TMB/H₂O₂ stained gel B). Note that a small amount of the HmuY-haem complex was formed after 24 h incubation of oxyhaemoglobin plus HmuY only (CBB stained gel track C and TMB/H₂O₂ stained gel track D), attributed to pickup of haem lost from oxyhaemoglobin as a result of auto-oxidation. The oxyhaemoglobin concentration was 16 µM (on a haemoglobin subunit basis), as was HmuY, whilst HRgpA was present at 0.4 µM.