Figure 9. HmuY-haem complex formation during the co-incubation of oxyhaemoglobin with both HmuY and Kgp.

HmuY and oxyhaemoglobin (both at 16 µM) were incubated at 37°C with Kgp (0.2 µM) and sampled periodically, and subjected to native PAGE. Gel tracks were loaded with ∼12 µg total protein.