Figure 12. Spectroscopic demonstration of the formation of the HmuY-ferrihaem complex during co-incubation of HmuY and iron(III) protoporphyrin IX in the presence of human serum albumin.
Panel A, spectra obtained during incubation of all three components. Panel B, incubation of albumin and haem. All components were present at 16 µM. See text for details.