Table 1.

Experimental protocol and the histopathological scores of intraosseous acute inflammation in bones

Groups	Animal no.	Dose	Time from inoculation to euthan-asia	IAI scores in selected bones of the right front leg
		CFU/kg BW	Days	Radius	Ulna	Os metacarpale III	Os metacarpale IV	Phalanx proximalis III	Phalanx proximalis IV	Phalanx medialis III	Phalanx medialis IV	Phalanx distalis III	Phalanx distalis IV
A	1	0	15	0	0	0	0	0	0	0	0	0	0
	2	0	15	0	0	0	0	0	0	0	0	0	0
B	3	5	14	0	0	0	0	0	0	0	0	0	0
	4	5	14	0	0	0	0	0	0	0	0	0	0
C	5	50	08*,**	0	0	0	0	0	0	0	0	0	0
	6	50	14	0	0	0	0	0	0	0	0	0	0
D	7	500	08*,**	0	0	0	0	1	0	0	0	0	0
	8	500	13	0	0	1	1	0	0	0	0	0	0
E	9	5000	13	1	1	1	0	1	0	0	0	0	0
	10	5000	05*	1	1	2	0	2	1	4	1	4	1
F	11	50 000	05*,**	4	1	0	3	4	0	1	0	0	0
	12	50 000	05*	0	0	4	1	1	0	0	0	0	0

IAI, intraosseous acute inflammation.

The scores of IAI are reported with Arabic numbers (0–4): 0, Not present; 1, Minimal to mild inflammation with no intramedullary abscess; 2, Moderate to severe inflammation with no intramedullary abscess; 3, Minimal to mild inflammation with intramedullary abscess; 4, Moderate to severe inflammation with intramedullary abscess.

^*Animals killed due to lameness.

^**Phlegmon formation.