Fig. 2.

The microaerobic growth of Bacillus anthracis under different culture conditions. (a) Static cultures grown at 37°C, 5% CO₂ in the nitrate-rich CSFM (triangles), Luria broth (circles), DMEM/F-12 (squares), and DMEM/F-12 supplemented with 300 μM NaNO₃ and 1 μg mL⁻¹ of BSA (diamonds) generate acidic and toxic Sups. Open symbols, dSterne; closed symbols, Sterne 34F2. (b and c) Aeration by shaking at 200 r.p.m. of the B. anthracis cultures grown at 37°C, 5% CO₂ in CSFM reduces the toxicity and acidification of Sups (b) and abrogates the consumption of nitrate from the medium (c). The final pH or the cultures is shown in (b). Error bars represent 95% confidence interval of mean.