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TLK1 is phosphorylated by Chk1 in response to hypoxia. (A) RKO cells were treated with the Chk 1 inhibitor UCN-01 (dose) and exposed to hypoxia (0.02% O2) as indicated. HU was used as a positive control. The levels of both phosphorylated and total TLK1 are shown. (B) RKO cells were treated with the Chk 1 inhibitor Gö6976 (100 nM) and exposed to hypoxia (0.02% O2) as indicated. HU was used a appositive control. The levels of both phosphorylated and total TLK1 are shown.
