Figure 2.

MMP-3 is a transcriptional target of Wnt signaling. (A) Northern blot analysis of total RNA extracted from C57MG and C57MG/Wnt1 clones (20 µg/lane) (top and middle). Staining of the gel with ethidium bromide (bottom). (B) Dual luciferase reporter assay for C57MG, C57MG/Wnt1 and C57MG cells treated with Wnt3a, 48 h after transfection with pGL2-SL1-luc plasmid. The data are representative of five experiments performed in triplicate. (C) Luciferase assay for the induction of Topflash reporter by MMP-3 and Wnt3a in the presence or absence of AG3340 (42 µg/ml). CT7 cells were co-cultured for 18 h with C57MG control cells, or C57MG/MMP-3 cells, or L/Wnt3a cells, or a combination of those three cell types. The data represent the mean (±SD) of four determinations from a representative experiment.