Figure 2.

Mature polyA^– hTR expressed in yeast interacts with all four core proteins of H/ACA snoRNPs. Immunoprecipitation experiments were carried out using IgG–Sepharose and extracts from strains expressing hTR and either Cbf5pZZ (lanes 1 and 2), Gar1pZZ (lanes 3 and 4), Nhp2pZZ (lanes 5 and 6), Nop10pZZ (lanes 7 and 8) or ZZNop1p (lanes 9 and 10). RNAs extracted from one-tenth of the input extracts (I) or from the pellets following immunoprecipitation (P) were separated on a 6% polyacrylamide gel and transferred to a nylon membrane. RNAs indicated on the left were detected by hybridization with antisense oligonucleotide probes.