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. 2001 Feb 1;29(3):598–603. doi: 10.1093/nar/29.3.598

Figure 3.

Figure 3

Accumulation in S.cerevisiae of mature polyA– hTR requires the presence of Cbf5p, Nhp2p and Nop10p. GAL::cbf5 (lanes 1–5), GAL::gar1 (lanes 6–10), GAL::nhp2 (lanes 11–15) and GAL::nop10 (lanes 16–20) strains transformed with phTR were grown in galactose-containing medium (lanes 1, 6, 11 and 16) and were then transferred to glucose-containing medium for 12, 24, 48 or 72 h. At each time-point, culture samples were collected from which total RNAs were extracted. These were separated on 6% polyacrylamide gels and transferred to nylon membranes. RNAs indicated on the left were detected by hybridization with specific oligonucleotide probes.