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. 2001 Feb 1;29(3):598–603. doi: 10.1093/nar/29.3.598

Figure 4.

Figure 4

Accumulation in S.cerevisiae of polyadenylated forms of hTR and of polyA– mature TLC1 does not require the presence of Nhp2p. The GAL::nhp2 strain transformed with phTR was grown in galactose-containing medium (lanes 1–3) and was then transferred to glucose-containing medium for 12, 24, 48 and 72 h. Culture samples were collected from which total RNAs were extracted (Tot). Samples of the total RNA preparations were incubated with biotinylated oligo(dT) and polyadenylated RNAs were precipitated using streptavidin-coated magnetic beads. RNAs were recovered from the supernatants (polyA–) or the pellets (polyA+). All RNAs were separated on a 6% polyacrylamide gel, transferred to a nylon membrane and hybridized with antisense oligonucleotide probes detecting TLC1, hTR, snR37 or U3 RNA.