Figure 2.

Effects of ace-HF on APP mRNA and protein levels. (A), RT-PCR analysis of transfected APP695sw mRNA expression in HEK293 cells. HEK293 cells were transfected with the plasmid DNA pcDNA-APP₆₉₅sw using the Calcium Phosphate Transfection System. Twelve hours after the transfection, cells were treated with 100 μol/L ace-HF; (B), Western blot analysis of APP protein in HEK293 APP Swedish cells probed with antibody 22C11. The cells were treated in the same way as (A); (C), RT-PCR analysis of endogenous APP mRNA expression in SH-SY5Y cells treated with different ace-HF concentration; (D), Western blot analysis of endogenous APP protein level in SH-SY5Y cells probed with antibody 22C11.Cells were treated with ace-HF at concentrations of 0, 0.1, 1, 10 and 100 μol/L for 12 h.