Figure 3.

ELISA assay of extracellular sAPPα. Data are presented as a percentage of control (DMSO) (*P< 0.05, n=4). (A), Transfected experiment for sAPPα level in HEK293 APP Swedish cells. HEK293 cells were transfected with the plasmid DNA pcDNA-APP₆₉₅sw using the Calcium Phosphate Transfection System. Twelve hours after the transfection, cells were treated with 100 μmol/L ace-HF for 12 h. (B), sAPPα level of endogenous APP processing in SH-SY5Y cells treated with ace-HF at concentrations of 0, 0.1, 1, 10 and 100 μmol/L ace-HF for 12 h.