Figure 4.
(A), Effects of the PKC inhibitor Calphostin C on ace-HF-stimulated sAPPα secretion from SH-SY5Y cells. Cells were co-treated with 50 αmol/L ace-HF and 0.5 αmol/L Calphostin C for 12 h. Then ELLISA assay was performed to detect sAPPα level. Data are presented as a percentage of control (DMSO) (*P< 0.05, ** P< 0.001,n=5). (B), Effects of ace-HF on α-secretase activity in SH-SY5Y cells. Cells were treated with 50 μmol/L ace-HF for 12 h. Then ELLISA assay was performed to detect α-secretase activities (** P< 0.001, n=3).