Figure 3.

Effects of Nodal and ALK7-ca on breast cancer cell growth. MDA-MB-231 (A, B) and MCF-7 (C, D) cells were transfected transiently with pcDNA4 (EV), Nodal, or ALK7-ca. At 48h post-transfection, proteins were extracted and probed with anti-c-myc antibody to confirm the expression of transgenes, anti-phosphoSmad2 to confirm the activation of Smad2, and anti-β-actin as a loading control (A, C). The number of metabolically active cells was measured by MTT assays (B, D). Data represent means ± SEM (n=8 wells). Experiments have been repeated twice and similar results obtained. * p<0.05 vs. the respective pcDNA control.