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. 2009 Feb 27;1(1):83–96.

Figure 4.

Figure 4

Nodal and ALK7-ca inhibited cell proliferation. A, B) BrdU assays. MDA-MB-231 (A) and MCF-7 (B) cells were transfected with pcDNA4, ALK7-ca, ALK7-kd, Nodal (N) or Nodal plus ALK7-kd. BrdU assays were performed at 48h after transfection. C) RT-PCR measurement of p21, p27, and BRCA2 mRNA levels. Total RNA was extracted from pcDNA4 (EV), Nodal (N) and ALK7-ca (CA) transfected cells at 6 and 24h after transfection and RT-PCR performed. A typical PCR gel picture was shown along with graphs showing quantified data (means ± SEM of three independent transfection and RT-PCR experiments). Different letters denote statistical significance (P<0.05).