Skip to main content
. 2009 Feb 27;1(1):83–96.

Figure 6.

Figure 6

Effects of Nodal and ALK7-ca on apoptosis of caspase 3-expressing MCF-7 cells. Control (MCF7/neo) or caspase 3-expressing MCF-7 (MCF7/Casp3) cells were transfected with Nodal (N), ALK7-ca (CA) or their empty control vector (EV) and 48h later, mRNA and protein extracted and TUNEL assays performed. A) RT-PCR detected the expression of caspase 3 in MCF7/Casp3 cells. B) Western blot showed the expression of caspase 3 in MCF7/Casp3 cells and the down regulation of Bcl-2 by Nodal and ALK7-ca in both cell lines. C) TUNEL assays revealed similar effects of Nodal and ALK7-ca on apoptosis between the control and MCF-7/Casp3 cells. *, p<0,05 vs the respective control.