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Caspase 3 inhibitor blocked Nodal induced apoptosis. TUNEL assay was performed after MDA-MB-231 cells were incubated with recombinant mouse Nodal (rmNodal, 500 ng/ml) for 48 h with or without 2 hour pretreatment with a caspase 3 inhibitor, Z-DEVD-FMK. Data represent Mean±SEM (n=3). Different letters denote statistical significance.
