Figure 2.

Coexpression of CGI-58 facilitates LD degradation by ATGLΔHD mutant. HeLa cells were treated with human ATGL siRNA and then transfected with each of the murine ATGL variants (wild type, ΔHD or ΔHD/S47A) in the presence of Myc-CGI58. After 18 h of incubation with 400 µM of oleic acid followed by 1 h of nutrient starvation, immunofluorescence staining was performed by using ATGL and Myc antibodies. Lipid droplets were co-stained with BODIPY 493/503 fluorescence dye.