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. 2010 Jul 15;9(14):2866–2877. doi: 10.4161/cc.9.14.12363

Figure 3.

Figure 3

Reduced duplex DNA degradation in Mirin-treated nuclear extracts. (A) 5′Cy3-labeled Template degradation products following gel separation. A duplex DNA substrate with a 5′Cy3-labeled Template was incubated with nuclear extracts from WI-38VA13 (CF), AT5BIVA (AT) and respective nuclear extracts treated with 1 mM Mirin. Degradation was allowed to proceed for 5, 10 or 15 min. A reaction with duplex only (Lane 1) in the absence of nuclear extract was included as control and incubated for 15 min. (B) Plot for % intensities of Full-length products. Full products in (A) were quantified and expressed as percent intensity (% intensity = (product intensity/total intensity) × 100). (C) 3′Cy3sp-labeled Top Strand degradation products following gel separation. A duplex DNA substrate with a 3′Cy3sp-labeled Top Strand was incubated with nuclear extracts from WI-38VA13 (CF), AT5BIVA (AT) and respective nuclear extracts treated with 1 mM Mirin. Degradation was allowed to proceed for 5, 10 or 15 min. A reaction with duplex only (Lane 1) in the absence of nuclear extract was included as control and incubated for 15 min. (D) Plot for % intensities of Full-length products. Full products in (A) were quantified and expressed as percent intensity (% intensity = (product intensity/total intensity) × 100).