Table 2.

Oligonucleotides used to assess DNA degradation

Name	Length (nt)	Sequence
Top strand	76	5′ACCCAG AGC TCG GTA CCC GGG GAT CCT CTA GAG TCG ACC TGC AGG CAT GCA AGC TTG GCA CTG GCC GTC GTT TTA C
Cy3 Template	71	3′CTC GAG CCA TGG GCC CCT AGG AGA TCT CAG CTG GAC GTC CGT ACG TTC GAA CCG TGA CCG GCA GCA AAA TG/Cy3
Cy3Sp Top Strand	50	5′ACCCAA GTC GAC CTG CAG GCA TGC AAG CTT GGC ACT GGC CGT CGT TTT AC/Cy3sp
Template	45	3′TCA GCT GGA CGT CCG TAC GTT CGA ACC GTG ACC GGC AGC AAA ATG

Underlined nucleotides are unpaired in the final substrate. Bold nucleotides are linked by phosphorothioate linkages.