Table 1.
Temperature dependence of mitfa alleles
| temp. | embryonic melanocytes(1) | adult melanocytes(2) | ||
|---|---|---|---|---|
| mitfavc7 | mitfafh53 | temp. range | mitfafh53 | |
| 23 | +* (n=7) | + | 25.0 – 26.9 | regular stripes (n=5) |
| 24 | +* (n=10) | +* (n=5) | 26.9 – 27.5 | stripes have fewer melanocytes (n=6) |
| 25 | +* (n=6) | +* (n=9) | ||
| 27 | +* | gaps (n=6) | 28.6 – 29.5 | very few melanocytes (< 50/side) (n=7) |
| 28.5 | 1–10 mels/emb. (n=9) | 10–50 mels/emb (n=6) | 31.0 – 31.8 | no melanocytes, solid xanthophore field (n=4) |
| 30 | none (n=8) | none (n=6) | ||
| 32 | none (n=4) | none (n=4) | ||
(1)
Embryos scored at ~ 80 hours post-fertilization, after shifting to indicated temperatures at ~ 10 hpf.
(2)
Fish from heterozygous intercrosses were were reared at restrictive temperatures for 3 days, mutant embryos selected and returned to 25 degrees for a further 10 days. They were then shifted to aquaria with individual heaters for 5 weeks, and scored for qualitative defects. Temperature in each tank was then recorded daily, and temperature range over the course of experiment shown
*
Full pattern development slower at these temperatures