Western blot analysis of sAPPα shows that treatments of cells with cis-2-Eicosenoic acid (20:1) (A, left) and cis-5, 8, 11-Eicosatrienoic acid (20:3) (B, left) did not affect sAPPα secretion to medium from SH-SY5Y cells. PMA treatment was used as a positive control. cis-2-Eicosenoic acid (20:1) (A, right) and cis-5, 8, 11-Eicosatrienoic acid (20:3) (B, right) did not affect membrane fluidity of cells, as the fluorescent intensity of FCVJ-labeled cells remained unchanged after treatments. Data are expressed as mean ± SD from at least three independent experiments (** p< 0.01 compared with control).