Table 3.
Target gene enrichment network analysis results
| Network/pathway | Gene number | Target genes | Enrichment P-value |
|---|---|---|---|
| KEGG a | |||
| Colorectal cancer | 3 | IGF1R, MET, FZD7 | 0.00056 |
| Focal adhesion | 3 | IGF1R, ITGB8, MET | 0.00653 |
| Adherens junction | 2 | IGF1R, MET | 0.00942 |
| Cell adhesion molecules (CAMs) | 2 | NEGR1, ITGB8 | 0.0256 |
| Ingenuity pathway analysis b | |||
| Genetic Disorder, Immunological Disease, Cardiovascular System Development and Function |
16 |
ASH1L, BAZ2A, DHX15, DMD, ELL2, ETS1, GABBR2, HIPK2, IGF1R, IGFBP5, MARCKS (includes EG:4082), MECP2, MET, PDGFA, SMARCA4, THRB |
30 |
| Organ Morphology, Reproductive System Development and Function, Cellular Development |
13 |
C7ORF42, CAPRIN1, CNOT1, DENR (includes EG:8562), GREM2, ITGB8, LARP4, NAB1, PHF15, SPOPL, TCF12, ZIC1, ZRANB2 |
25 |
| Cell Signaling, Cancer, Cell Cycle | 12 |
AFF2, AFF4, AKIRIN2, DDX3X, FOXN3, PTPRD, PTPRS (includes EG:5802), SFRS1, SGIP1, TNPO1, YTHDC1, ZNF148 |
23 |
| Organismal Development, Gene Expression, Cancer |
12 |
ARHGEF3, CUL3, FZD7, GATAD2B, GPR85,MNT, PDIK1L, PRDM16, SOX4, SOX11, ZBTB34, ZFHX4 |
22 |
| Cell Morphology, Cellular Assembly and Organization, Cell Cycle |
9 |
AAK1, AZIN1, GPR158, HNRNPU,NEGR1, NUFIP2, PXMP3, RNF165, WDR40A |
16 |
Putative gene targets from Targetscan (http://www.targetscan.org) that are related to 2 or more differential expressed miRNAs were analyzed using web-based GEne SeT AnaLysis Toolkit (WebGestalt). Significance of the pathways was determined by a p value <0.05 and presence of at least 2 target genes in the pathway.
The networks were generated through the use of Ingenuity Pathways Analysis ngenuity® Systems, www.ingenuity.com). Each gene identifier was mapped to its corresponding gene object in the Ingenuity Pathways Knowledge Base (IPKB) and overlaid onto a global molecular network developed from information contained in the IPKB. Enrichment p-values correspond to network score (negative log of p-values of Fisher test). Genes listed are genes that are targeted by two or more microRNAs that are differentially expressed in preeclamptic placenta in our study.