Table 2.
Comparison of Data Acquisition and Analysis Method Described Herein with Other Published Methods
| Ramm (Ref. #24) | Price (Ref. #25) | Mitchell (Ref. #4) | Radio (Ref. #5) | Yeyeodu (This paper) | |
|---|---|---|---|---|---|
| Instrument | IN Cell Analyser 1000 (Amersham) | Automated microscope stage (Prior Scientific) | Ludl stage | ArrayScan V (Cellomics) | BD Pathway 855 |
| Acquisition software | (included) | (Media Cybernetics) | VolumeScan (Vaytek) | (included) | BD AttoVision™ 1.6 |
| Autofocus | yes | yes | yes | yes | yes |
| Image intensity resolution | 12 bit | NR | 24 bit | NR | 12 bit |
| Analysis software | (included) | custom algorithm (Advansoft) in ImagePre Plus 4.1 (Media Cybernetics) | custom algorithm (detailed in text) in ImagePro Plus | (included) | BD AttoVision™ 1.6 with Neurite Outgrowth Module |
| Neurite defined | ~2x cell diam. (30µm) | NR | NR | ~2x total neurite length in cells w/o NGF (> 20 µm) | >1.5x cell width |
| Parameters measured | |||||
| Total neurite length | yes | yes | yes | ||
| Total cell body area | yes | ||||
| Num. neurites | yes | yes | |||
| Avg cell diameter | yes | ||||
| Num. nuclei or cells | yes | yes | yes | yes | |
| Individual neurite length | yes | ||||
| Num. branches | yes | yes | yes | ||
| Maximum neurite length | yes | yes | |||
| Neurites/cell | yes | ||||
| Cell body area | yes | ||||
| Parameters derived | |||||
| % neurite bearing cells | yes | yes | |||
| Num. cells | yes | yes | |||
| Mean neurite length | yes | yes | yes | yes | |
| Total neurite length/cell | yes | yes | yes | ||
| Mean neurite length/cell | yes | yes |
NR=not reported.