Table 3.

Comparison of Sample Preparation and Assay Conditions

	Ramm (Ref. #24)	Price (Ref. #25)	Mitchell (Ref. #4)	Radio (Ref. #5)	Yeyeodu (This paper)
Detection method(s)	Abs: primary + FITC-secondary	Coomassie blue G-250	Hoechst + Ab: primary + AlexaFluor + 488-secondary	Hoechst + Ab: primary + AlexaFluor + 488-secondary	a) Hoechst + GFP expressing cells or b) Hoechst + CellMask Red dye
Plate/pretreat	8 h	5 d	4-6 d	1-4 d	16-24 h
Treat	3 d	4 d	16-24 h	4 d	2 d
Total time in culture	>3 d	9 d	>4.5 d	5 d	<3 d
Cell preparation for imaging
Fix(/stain)	2 h	1 h	30 min	20 min	30 min
Permeabilize/wash	30-60 min	-	30 min	30 min	-
Primary Antibody	1 h	-	18 h	1 h	-
Secondary Antibody	1 h	-	1 h	1 h	-
Final stain	-	ON	-	-	-
Total preparation time	4.5-5 h	ON	ON	3 h	30 min

ON=overnight; Abs=Antibodies; d=day(s).