Table 1. Distribution of mutants obtained in the three oligonucleotide libraries.
Clone genotype |
Library I, α = 49.5% |
Library II, α = 78.9% |
Library III,
α = 10.6% |
Wild-type | 7 | 1 | 27 |
Single mutants | 5 | 2 | 11 |
Double mutants | 13 | 6 | 5 |
Triple mutants | 8 | 9 | 0 |
Quadruple mutants | 2 | 18 | 0 |
Deletions | 5 | 1 | 4 |
Insertions | 2 | 1 | 2 |
Total | 42 | 38 | 49 |