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Single-cell electrophysiology. (A) Neurons derived from Cyno-1 express voltage-dependent inward currents that are blocked by tetrodotoxin. Currents were elicited by membrane depolarizations to 0 mV every 15 s from a holding potential of -70 mV. Application of 0.5 μM tetrodotoxin inhibited >90% of these currents. (B) Inhibition was complete within 30 s of tetrodotoxin application and washed completely in <1 min.
