Fig. 2.

Failure in secondary palate formation and hypoplasia of lungs in Gpr124-deficient embryos. All Gpr124-deficient embryos had a large cleft of the secondary hard palate (B), which is normally fused (A), at P0. Yellow arrowheads point to the fused midline in A and to the unfused medial edges of the palatal shelves in B. Visualization of embryonic morphology by soft tissue–enhanced microcomputed X-ray tomography (μCT) at E15.5 revealed that, although other internal organs appeared normal, lungs (shaded green, arrows) were hypoplastic in Gpr124-deficient embryos (D) compared with control littermates (C).