Fig. 4.

Tbx15 overexpression decreases mitochondrial mass of 3T3-L1 preadipocytes. Total mitochondrial mass was measured by fluorescent staining, using 50 nM MitoTracker Green FM, of control (white bar) or 3T3-L1-Tbx15 (black bar) preadipocytes (A) and adipocytes differentiated for 8 d in the presence of 1 μM rosiglitazone (B). Fluorescence was analyzed by FACS on a population of 200,000 cells. Values are means ± SEM of triplicates of three separate experiments. Differences were statistically analyzed by Student's t test (two-way, unequal variance). *P < 0.05.