Fig. 2.

Characterization of Cx43 phosphorylation and binding partners association. (A Upper) Immunoprecipitation analysis showing the association level of N-cad, ZO1, and c-Src with Cx43 in WT and mdx heart. (Lower) Association between c-Src and Cx43 in WT and mdx heart. Densitometric analyses are shown in Right. *P < 0.05. (B) Confocal analysis of c-Src (green) and Cx43 (red) distribution in WT and mdx heart. Nuclei were counterstained with TOPRO3 (blue). (Scale bar, 50 μm.) (C) Western blotting analysis showing phosphorylation level of c-Src (Y418) and Cx43 (S262, Y265, S368, and S255). Right shows band density. *P < 0.05.