Fig. 4.

Acetylation alters Cx43 intracellular distribution. (A) Confocal analysis of NIH 3T3 cells transient-transfected with mock (A and B), WT (Cx43^WT; C and D), or K → A (Cx43^3A; E and F) and K → Q (Cx43^3Q; G and H) connexin 43 expression vectors. Samples were analyzed before (A, C, D, and G) and after TSA treatment (B, D, F, and H). Insets show enlarged details representative of each experimental condition. Transfection efficiency was 80% on average for each vector. Experiments were repeated three times. (B) Quantitative evaluation of Cx43^WT, Cx43^3A, and Cx43^3Q distribution at cell to cell junctions (Left) or the nuclear level (Right). Data are expressed as mean fluorescence intensity (MFI); about 300 cells were counted for each experimental condition. *P < 0.05 vs. Cx43^WT in untreated cells; ^§P < 0.05 vs. Cx43^WT in untreated cells.