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. 2011 Jan 31;108(7):2795–2800. doi: 10.1073/pnas.1013124108

Fig. 5.

Fig. 5.

HDACs localization, function, and association with Cx43. (A) Confocal analysis of Cx43 (red) and HDAC4 (green; Top), HDAC5 (green; Middle), or HDAC3 (green; Bottom) expression and distribution in the WT and mdx heart. Nuclei were counterstained with TOPRO3 (blue). (Scale bar, 50 μm.) Enlarged details are shown in the far right column. (Scale bar, 50 μm.) (B) Coimmunoprecipitation experiments showing HDAC4, -5, and -3 association with Cx43 in the WT and mdx heart. Relative densitometries are shown in Right. *P < 0.05. (C) HDAC4-specific activity evaluated in the WT and mdx heart samples. *P < 0.05. (D) Confocal analysis of N-cad (green) and Cx43 (red) expression and distribution in hearts from WT treated with MC1568 or equivalent amounts of solvent. Nuclei were counterstained with TOPRO3 (blue). (Scale bar, 50 μm.) The graph shows Cx43 and N-cad percentage of colocalization. *P < 0.05. Lower shows the acetylation level of Cx43 in WT treated with MC1568 or an equivalent amount of solvent (Left). Densitometry is shown to Right. *P < 0.05.

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