HdhSTOP/– embryos
resemble Hdh–/– embryos.
Examples of Hdh–/– (A and D), HdhSTOP/– (B and E), wild-type (C)
and Hdh+/– (F) embryos at E8.5 (A–C) and E9.5 (D–F) are shown. The embryonic portions (e) of the Hdh–/– and HdhSTOP/– mutants are stubby and retarded in development in comparison to their wild-type
counterparts. The extraembryonic (exe) portion of the mutant embryos
and the yolk sac (ys) of the E8.5 wild-type embryo are indicated.
The ectoplacental cone (epc) of the embryos in (A), (B), (D) and
(E) was not removed during dissection, while the yolk sac was removed
from the embryo in (F) for better visibility. Reichert’s
membrane (r) is visible as a thin transparent sac surrounding the
mutant embryos in (A), (B) and (D) but was removed during dissection
from the embryos in (C) and (E).