Fig. 1.
Deletion of 10 T3E genes in CUCPB5500 yields DC3000D28E (CUCPB5585), which is strongly reduced in growth in N. benthamiana. Bacteria were inoculated with a blunt syringe at 3 × 104 cfu/mL in MgCl2 buffer, and populations in three 0.8-cm leaf disks were determined at 6 dpi. The arithmetic mean of log-transformed values of four replicate infiltrations from independent plants is displayed ± SD. Strains with the same letter are not statistically different based on a Tukey's honestly significant difference (HSD) test (α = 0.05). The cells in the genotype grid are shadowed in dark gray if the corresponding strains carry a deletion of the matching genomic region or are left white if the locus is WT. The Ω for hopY1 indicates that the gene was interrupted by insertion of a spectinomycin resistance cassette flanked by FRT sites. This experiment was repeated three times with similar results.