Fig. 3.

Restoration to native loci in DC3000D28E of genes encoding the AvrPto and AvrE REGs suggests that AvrPto and AvrPtoB interfere at an early phase of the host immune response. White fill in the genotype grid indicates that the locus was restored (note that the CEL includes hopM1). Strains harboring combinations of multiple WT loci were constructed by sequential integrations. Growth assays were performed at 6 dpi as in Fig. 1 with means ± SD of the bacterial populations calculated using values from three replicate leaves per strain. Means with the same letters are not statistically different based on a Tukey's HSD test (α = 0.05). This experiment was repeated three times with similar results.