Fig. 6.

In utero flutamide exposure inhibits bud elongation but has little if any effect on bud specification or initiation in the ventral UGS of wild-type males. A,B: SEM images of ventral UGE from a control (A) and a flutamide-exposed (B) mouse on the day of birth. Ventral buds are tinted orange. One prostatic bud broke off during sample preparation; its base is outlined in black. Both images are ventral views, taken with samples in the same orientation and at the same nominal magnification. C: Bud count data and histograms of ventral bud distribution on the left-right axis. Histograms were prepared by plotting the total number of ventral buds, from all replicate samples, present in each of 20 equal segments along the left-right UGE axis. For purposes of comparison, ventral bud distribution histograms from both control and flutamide-exposed mice are shown. Ventral bud numbers for the flutamide-exposed group are shown as mean ± SE, with the number of replicate samples used to determine bud number in parentheses. Bud distribution was plotted from fewer samples (n = 6) because 2 UGEs were not oriented favorably.